| Rank | Player | Date | Opponent | Assists
|
|---|
| 1
| Jenalyn Yumol | Feb 20, 2016 | Nipissing | 14 |
| 2
| Angeline Campbell | Nov 15, 2024 | Ottawa | 10 |
| 3
| Ana Caldeira Rua | Oct 25, 2017 | Western | 10 |
| 4
| Melissa Tatti | Nov 16, 2018 | Toronto | 10 |
| 5
| Melissa Tatti | Jan 11, 2020 | Toronto | 10 |
| 6
| Melissa Tatti | Jan 24, 2020 | Ontario Tech | 10 |
| 7
| Madalyn Weinert | Feb 17, 2024 | Nipissing | 10 |
| 8
| Madalyn Weinert | Jan 31, 2025 | Lakehead | 10 |
| 9
| Angeline Campbell | Feb 2, 2024 | Windsor | 9 |
| 10
| Melissa Tatti | Feb 5, 2016 | Toronto | 9 |
| 11
| Melissa Tatti | Nov 24, 2018 | Ottawa | 9 |
| 12
| Melissa Tatti | Jan 8, 2020 | York | 9 |
| 13
| Madalyn Weinert | Nov 25, 2023 | McMaster | 9 |
| 14
| Madalyn Weinert | Jan 5, 2024 | Toronto Metropolitan | 9 |
| 15
| Jenalyn Yumol | Nov 28, 2015 | Lakehead | 9 |
| 16
| Oluwatito Akinnusi | Jan 27, 2024 | Lakehead | 8 |
| 17
| Devyn Cuncic | Feb 11, 2011 | Lakehead | 8 |
| 18
| Jenneke Pilling | Nov 23, 2019 | Laurentian | 8 |
| 19
| Ana Caldeira Rua | Oct 28, 2017 | Laurier | 8 |
| 20
| Melissa Tatti | Feb 4, 2017 | Laurier | 8 |
| 21
| Melissa Tatti | Nov 8, 2019 | Guelph | 8 |
| 22
| Melissa Tatti | Nov 9, 2019 | Algoma | 8 |
| 23
| Melissa Tatti | Nov 22, 2019 | Nipissing | 8 |
| 24
| Melissa Tatti | Feb 8, 2020 | Waterloo | 8 |
| 25
| Madalyn Weinert | Jan 27, 2023 | Nipissing | 8 |
|
|
| Rank | Player | Date | Opponent | Assists
|
|---|
| 26
| Madalyn Weinert | Nov 9, 2024 | Waterloo | 8 |
| 27
| Jenalyn Yumol | Jan 9, 2016 | Windsor | 8 |
| 28
| Jenalyn Yumol | Feb 3, 2016 | Ryerson | 8 |
| 29
| Jenalyn Yumol | Feb 24, 2016 | McMaster | 8 |
| 30
| Jamie Addy | Jan 18, 2026 | Lakehead | 7 |
| 31
| Bridget Atkinson | Feb 15, 2017 | Western | 7 |
| 32
| Noor Bazzi | Nov 4, 2023 | York | 7 |
| 33
| Devyn Cuncic | Nov 14, 2009 | York | 7 |
| 34
| Devyn Cuncic | Jan 9, 2010 | Windsor | 7 |
| 35
| Devyn Cuncic | Nov 27, 2010 | Laurentian | 7 |
| 36
| Devyn Cuncic | Jan 19, 2011 | Waterloo | 7 |
| 37
| Jenna Gonneau | Jan 7, 2005 | RMC | 7 |
| 38
| Brooke-Lyn Murdoch | Jan 20, 2018 | Windsor | 7 |
| 39
| Brooke-Lyn Murdoch | Feb 17, 2018 | Windsor | 7 |
| 40
| Britt Pegg | Feb 6, 2010 | McMaster | 7 |
| 41
| Rebecca Ralph | Nov 27, 2015 | Lakehead | 7 |
| 42
| Melissa Tatti | Nov 28, 2015 | Lakehead | 7 |
| 43
| Melissa Tatti | Jan 13, 2017 | Lakehead | 7 |
| 44
| Melissa Tatti | Feb 9, 2019 | Western | 7 |
| 45
| Melissa Tatti | Nov 2, 2019 | Toronto | 7 |
| 46
| Melissa Tatti | Nov 15, 2019 | Lakehead | 7 |
| 47
| Melissa Tatti | Nov 29, 2019 | McMaster | 7 |
| 48
| Melissa Tatti | Jan 29, 2020 | York | 7 |
| 49
| Melissa Tatti | Feb 14, 2020 | Ottawa | 7 |
| 50
| Madalyn Weinert | Nov 24, 2023 | Queen's | 7 |
|