| Rank | Player | Date | Opponent | Assists
|
|---|
| 1
| Ryan Steer | Feb 27, 2008 | Western | 11 |
| 2
| Ryan Steer | Feb 28, 2007 | Guelph | 9 |
| 3
| Khalid Abdel-Gabar | Mar 14, 2015 | Saskatchewan | 8 |
| 4
| Khalid Abdel-Gabar | Mar 1, 2014 | McMaster | 7 |
| 5
| Khalid Abdel-Gabar | Feb 22, 2014 | Laurier | 6 |
| 6
| Kevin Kloostra | Mar 10, 2007 | Carleton | 6 |
| 7
| Shakael Pryce | Feb 19, 2020 | Laurentian | 6 |
| 8
| Mike Rocca | Feb 21, 2018 | Waterloo | 6 |
| 9
| Khalid Abdel-Gabar | Feb 25, 2015 | Toronto | 5 |
| 10
| Khalid Abdel-Gabar | Feb 28, 2015 | McMaster | 5 |
| 11
| Khalid Abdel-Gabar | Mar 13, 2015 | Bishop's | 5 |
| 12
| Corey Boswell | Feb 28, 2009 | McMaster | 5 |
| 13
| Alex Campbell | Mar 2, 2016 | Laurentian | 5 |
| 14
| Josh Collins | Mar 19, 2010 | Saskatchewan | 5 |
| 15
| Josh Collins | Mar 21, 2010 | Cape Breton | 5 |
| 16
| Monty Hardware | Mar 6, 2010 | Lakehead | 5 |
| 17
| Isiah Osborne | Mar 5, 2016 | McMaster | 5 |
| 18
| Chris Poloniato | Feb 19, 2020 | Laurentian | 5 |
| 19
| Ryan Steer | Mar 3, 2007 | McMaster | 5 |
| 20
| Ryan Steer | Mar 10, 2007 | Carleton | 5 |
| 21
| Greg Surmacz | Mar 10, 2007 | Carleton | 5 |
| 22
| Khalid Abdel-Gabar | Mar 6, 2015 | Ottawa | 4 |
| 23
| Khalid Abdel-Gabar | Mar 12, 2015 | Ryerson | 4 |
| 24
| Lorenzo Barbieri | Feb 25, 2023 | Guelph | 4 |
| 25
| Lorenzo Barbieri | Mar 1, 2023 | Ottawa | 4 |
|
|
| Rank | Player | Date | Opponent | Assists
|
|---|
| 26
| Corey Boswell | Mar 3, 2007 | McMaster | 4 |
| 27
| Corey Boswell | Mar 4, 2009 | Western | 4 |
| 28
| Najee Brown-Henderson | Mar 1, 2023 | Ottawa | 4 |
| 29
| Josh Collins | Feb 22, 2014 | Laurier | 4 |
| 30
| Enrico Diloreto | Mar 1, 2014 | McMaster | 4 |
| 31
| Ziphion Grant | Mar 16, 2022 | Lakehead | 4 |
| 32
| Isaac Kuon | Mar 6, 2010 | Lakehead | 4 |
| 33
| Evan Matthews | Mar 6, 2015 | Ottawa | 4 |
| 34
| Isiah Osborne | Mar 2, 2016 | Laurentian | 4 |
| 35
| Bradford Parker | Feb 25, 2015 | Toronto | 4 |
| 36
| Michael Petrella | Feb 23, 2013 | Brock | 4 |
| 37
| Lien Phillip | Feb 28, 2014 | Carleton | 4 |
| 38
| Mike Rocca | Mar 13, 2015 | Bishop's | 4 |
| 39
| Mike Rocca | Mar 12, 2016 | Ottawa | 4 |
| 40
| Mike Rocca | Feb 22, 2017 | McMaster | 4 |
| 41
| Greg Allin | Feb 28, 2007 | Guelph | 3 |
| 42
| Greg Allin | Mar 10, 2007 | Carleton | 3 |
| 43
| Richard Allin | Mar 3, 2007 | McMaster | 3 |
| 44
| Corey Boswell | Mar 16, 2007 | Brandon | 3 |
| 45
| Najee Brown-Henderson | Feb 25, 2023 | Guelph | 3 |
| 46
| Alex Campbell | Mar 12, 2015 | Ryerson | 3 |
| 47
| Josh Collins | Feb 27, 2010 | Laurier | 3 |
| 48
| Josh Collins | Mar 6, 2010 | Lakehead | 3 |
| 49
| Josh Collins | Mar 13, 2010 | Carleton | 3 |
| 50
| Josh Collins | Mar 20, 2010 | UQAM | 3 |
|