| Rank | Player | Date | Opponent | Assists
|
|---|
| 1
| Jenneke Pilling | Mar 26, 2022 | Ryerson | 7 |
| 2
| Mackenzie Robinson | Mar 23, 2022 | Western | 7 |
| 3
| Nicole Rosenkranz | Feb 23, 2013 | McMaster | 7 |
| 4
| Melissa Tatti | Mar 7, 2020 | UPEI | 7 |
| 5
| Jenneke Pilling | Mar 5, 2020 | Calgary | 6 |
| 6
| Jenneke Pilling | Mar 19, 2022 | McMaster | 6 |
| 7
| Jenneke Pilling | Mar 23, 2022 | Western | 6 |
| 8
| Melissa Tatti | Feb 20, 2019 | Laurier | 6 |
| 9
| Devyn Cuncic | Feb 24, 2010 | McMaster | 5 |
| 10
| Kristin Gallant | Feb 23, 2019 | McMaster | 5 |
| 11
| Jenneke Pilling | Mar 7, 2020 | UPEI | 5 |
| 12
| Andrea Polischuk | Feb 23, 2013 | McMaster | 5 |
| 13
| Mackenzie Robinson | Mar 31, 2022 | Acadia | 5 |
| 14
| Melissa Tatti | Feb 22, 2017 | Lakehead | 5 |
| 15
| Melissa Tatti | Mar 5, 2020 | Calgary | 5 |
| 16
| Madalyn Weinert | Feb 25, 2023 | Queen's | 5 |
| 17
| Madalyn Weinert | Feb 19, 2025 | Laurentian | 5 |
| 18
| Sofia Croce | Feb 29, 2020 | Ryerson | 4 |
| 19
| Devyn Cuncic | Feb 23, 2011 | Lakehead | 4 |
| 20
| Jessica Del Signore | Feb 23, 2011 | Lakehead | 4 |
| 21
| Kristin Gallant | Mar 5, 2020 | Calgary | 4 |
| 22
| Victoria Lawrence | Mar 31, 2022 | Acadia | 4 |
| 23
| Madison MacInnis | Feb 19, 2025 | Laurentian | 4 |
| 24
| Jessica Morris | Feb 29, 2020 | Ryerson | 4 |
| 25
| Jessica Morris | Mar 7, 2020 | UPEI | 4 |
|
|
| Rank | Player | Date | Opponent | Assists
|
|---|
| 26
| Jenneke Pilling | Feb 29, 2020 | Ryerson | 4 |
| 27
| Mackenzie Robinson | Mar 26, 2022 | Ryerson | 4 |
| 28
| Lauren Stewart | Feb 27, 2010 | Windsor | 4 |
| 29
| Melissa Tatti | Feb 23, 2019 | McMaster | 4 |
| 30
| Melissa Tatti | Feb 26, 2020 | Western | 4 |
| 31
| Melissa Tatti | Feb 29, 2020 | Ryerson | 4 |
| 32
| Melissa Tatti | Mar 8, 2020 | Saskatchewan | 4 |
| 33
| Nicola Thompson | Mar 1, 2002 | McMaster | 4 |
| 34
| Madalyn Weinert | Feb 22, 2023 | Windsor | 4 |
| 35
| Madalyn Weinert | Feb 24, 2024 | Toronto Metropolitan | 4 |
| 36
| Madalyn Weinert | Feb 22, 2025 | Toronto Metropolitan | 4 |
| 37
| Madalyn Weinert | Feb 21, 2026 | Windsor | 4 |
| 38
| Shailah Adams | Feb 18, 2026 | McMaster | 3 |
| 39
| Allison Addy | Mar 26, 2022 | Ryerson | 3 |
| 40
| Allison Addy | Feb 19, 2025 | Laurentian | 3 |
| 41
| Jamie Addy | Feb 18, 2026 | McMaster | 3 |
| 42
| Jamie Addy | Feb 21, 2026 | Windsor | 3 |
| 43
| Noor Bazzi | Feb 22, 2023 | Windsor | 3 |
| 44
| Angeline Campbell | Feb 19, 2025 | Laurentian | 3 |
| 45
| Kira Cornelissen | Mar 2, 2016 | Guelph | 3 |
| 46
| Sofia Croce | Feb 22, 2020 | Windsor | 3 |
| 47
| Sofia Croce | Feb 26, 2020 | Western | 3 |
| 48
| Devyn Cuncic | Mar 9, 2012 | Saskatchewan | 3 |
| 49
| Samantha De Jong | Feb 25, 2012 | Laurier | 3 |
| 50
| Jessica Del Signore | Feb 25, 2012 | Laurier | 3 |
|