| Rank | Player | Date | Opponent | Assists
|
|---|
| 1
| Emma Ritcey | Feb 24, 2018 | Ottawa | 9 |
| 2
| Emma Ritcey | Feb 19, 2020 | Laurier | 8 |
| 3
| Emma Ritcey | Feb 20, 2019 | York | 7 |
| 4
| Emma Weltz | Apr 2, 2022 | Winnipeg | 7 |
| 5
| Jenny Wright | Feb 22, 2014 | Toronto | 7 |
| 6
| Emma Ritcey | Feb 22, 2020 | Ryerson | 6 |
| 7
| Emma Weltz | Mar 11, 2023 | Alberta | 6 |
| 8
| Haley Barbieri | Mar 10, 2024 | Laval | 5 |
| 9
| Liz Boag | Feb 22, 2012 | Ryerson | 5 |
| 10
| Julia Chadwick | Feb 25, 2023 | Brock | 5 |
| 11
| Abby Dixon | Mar 9, 2017 | Cape Breton | 5 |
| 12
| Emily Hazlett | Mar 3, 2017 | Windsor | 5 |
| 13
| Emma Weltz | Apr 3, 2022 | Brock | 5 |
| 14
| Emma Weltz | Mar 1, 2023 | Waterloo | 5 |
| 15
| Emma Weltz | Mar 4, 2023 | Carleton | 5 |
| 16
| Jenny Wright | Mar 15, 2014 | Alberta | 5 |
| 17
| Liz Boag | Feb 23, 2013 | Ottawa | 4 |
| 18
| Liz Boag | Feb 25, 2015 | Laurentian | 4 |
| 19
| Julia Chadwick | Mar 12, 2023 | Carleton | 4 |
| 20
| Sophie de Goede | Mar 16, 2022 | York | 4 |
| 21
| Abby Dixon | Mar 5, 2016 | McMaster | 4 |
| 22
| Laura Donovan | Feb 28, 2024 | Toronto Metropolitan | 4 |
| 23
| Katie Guthrie | Feb 17, 2010 | Toronto | 4 |
| 24
| Emily Hazlett | Feb 28, 2015 | Laurier | 4 |
| 25
| Emily Hazlett | Mar 9, 2017 | Cape Breton | 4 |
|
|
| Rank | Player | Date | Opponent | Assists
|
|---|
| 26
| Bridget Mulholland | Feb 25, 2017 | Lakehead | 4 |
| 27
| Bridget Mulholland | Feb 25, 2023 | Brock | 4 |
| 28
| Bridget Mulholland | Mar 1, 2023 | Waterloo | 4 |
| 29
| Bridget Mulholland | Mar 9, 2023 | UQAM | 4 |
| 30
| Christine Wallace | Feb 22, 2012 | Ryerson | 4 |
| 31
| Emma Weltz | Mar 31, 2022 | Saskatchewan | 4 |
| 32
| Emma Weltz | Feb 24, 2024 | Guelph | 4 |
| 33
| Emma Weltz | Mar 2, 2024 | Carleton | 4 |
| 34
| Jenny Wright | Feb 20, 2013 | Ryerson | 4 |
| 35
| Marianne Alarie | Feb 24, 2018 | Ottawa | 3 |
| 36
| Isabella Belvedere | Mar 1, 2023 | Waterloo | 3 |
| 37
| Liz Boag | Feb 20, 2013 | Ryerson | 3 |
| 38
| Liz Boag | Feb 28, 2015 | Laurier | 3 |
| 39
| Julia Chadwick | Feb 19, 2020 | Laurier | 3 |
| 40
| Julia Chadwick | Apr 3, 2022 | Brock | 3 |
| 41
| Julia Chadwick | Mar 1, 2023 | Waterloo | 3 |
| 42
| Julia Chadwick | Mar 4, 2023 | Carleton | 3 |
| 43
| Julia Chadwick | Feb 24, 2024 | Guelph | 3 |
| 44
| Julia Chadwick | Feb 28, 2024 | Toronto Metropolitan | 3 |
| 45
| Julia Chadwick | Mar 7, 2024 | Saint Mary's | 3 |
| 46
| Sophie de Goede | Feb 20, 2019 | York | 3 |
| 47
| Sophie de Goede | Feb 22, 2020 | Ryerson | 3 |
| 48
| Abby Dixon | Feb 25, 2015 | Laurentian | 3 |
| 49
| Abby Dixon | Mar 2, 2016 | Laurentian | 3 |
| 50
| Abby Dixon | Feb 25, 2017 | Lakehead | 3 |
|