| Rank | Player | Date | Opponent | Assists
|
|---|
| 1
| Jenna Gonneau | Jan 7, 2005 | RMC | 7 |
| 2
| Kristin Gallant | Oct 28, 2017 | Laurier | 6 |
| 3
| Kristin Gallant | Nov 24, 2017 | McMaster | 6 |
| 4
| Kristin Gallant | Jan 27, 2018 | Waterloo | 6 |
| 5
| Kristin Gallant | Feb 3, 2018 | Waterloo | 6 |
| 6
| Kristin Gallant | Feb 10, 2018 | Lakehead | 6 |
| 7
| Dacia O'Sullivan | Jan 7, 2005 | RMC | 6 |
| 8
| Melissa Tatti | Feb 11, 2015 | Guelph | 6 |
| 9
| Shailah Adams | Nov 4, 2023 | York | 5 |
| 10
| Kaley DeMont | Nov 13, 2021 | Lakehead | 5 |
| 11
| Kristin Gallant | Jan 31, 2018 | Laurier | 5 |
| 12
| Melissa Tatti | Jan 17, 2015 | Laurentian | 5 |
| 13
| Hillary Woodside | Feb 2, 2008 | Windsor | 5 |
| 14
| Shailah Adams | Jan 5, 2024 | Toronto Metropolitan | 4 |
| 15
| Jamie Addy | Jan 12, 2024 | Algoma | 4 |
| 16
| Theresa Brown | Nov 11, 2022 | Lakehead | 4 |
| 17
| Theresa Brown | Jan 13, 2023 | Algoma | 4 |
| 18
| Kaley DeMont | Nov 24, 2021 | Guelph | 4 |
| 19
| Kyla Douros | Jan 7, 2005 | RMC | 4 |
| 20
| Kristin Gallant | Nov 17, 2017 | Nipissing | 4 |
| 21
| Christine Sirignano | Feb 12, 2016 | Carleton | 4 |
| 22
| Lauren Stewart | Nov 12, 2005 | RMC | 4 |
| 23
| Ava Stranges | Jan 17, 2025 | McMaster | 4 |
| 24
| Ava Stranges | Jan 24, 2025 | Ontario Tech | 4 |
| 25
| Ava Stranges | Jan 25, 2025 | Queen's | 4 |
|
|
| Rank | Player | Date | Opponent | Assists
|
|---|
| 26
| Melissa Tatti | Nov 14, 2014 | Algoma | 4 |
| 27
| Melissa Tatti | Jan 16, 2015 | Nipissing | 4 |
| 28
| Amanda Tofano | Jan 7, 2005 | RMC | 4 |
| 29
| Madalyn Weinert | Nov 3, 2021 | Laurier | 4 |
| 30
| Madalyn Weinert | Nov 20, 2021 | Windsor | 4 |
| 31
| Mikayla Williams | Nov 29, 2025 | Algoma | 4 |
| 32
| Hillary Woodside | Jan 19, 2008 | Windsor | 4 |
| 33
| Jenalyn Yumol | Nov 25, 2011 | RMC | 4 |
| 34
| Jenalyn Yumol | Feb 18, 2012 | Waterloo | 4 |
| 35
| Shailah Adams | Nov 3, 2023 | Toronto | 3 |
| 36
| Jamie Addy | Jan 20, 2024 | Ontario Tech | 3 |
| 37
| Theresa Brown | Nov 12, 2022 | Lakehead | 3 |
| 38
| Theresa Brown | Dec 4, 2022 | Ottawa | 3 |
| 39
| Theresa Brown | Jan 18, 2023 | McMaster | 3 |
| 40
| Devyn Cuncic | Nov 10, 2007 | RMC | 3 |
| 41
| Kristin Gallant | Nov 10, 2017 | Toronto | 3 |
| 42
| Kristin Gallant | Nov 18, 2017 | Laurentian | 3 |
| 43
| Alicia Higgins | Nov 24, 2021 | Guelph | 3 |
| 44
| Madison MacInnis | Nov 24, 2021 | Guelph | 3 |
| 45
| Annie McNeely | Nov 12, 2010 | RMC | 3 |
| 46
| Audrey Ntetani | Feb 12, 2022 | Windsor | 3 |
| 47
| Sarah Pallett | Nov 7, 2014 | Carleton | 3 |
| 48
| Sarah Pallett | Nov 14, 2014 | Algoma | 3 |
| 49
| Sarah Pallett | Jan 16, 2015 | Nipissing | 3 |
| 50
| Stephanie Parfitt | Feb 2, 2008 | Windsor | 3 |
|